0.25% Trypsin-EDTA, Phenol Red (modified)

Product Description

In vitro culture of tissue cells and tissue cells dispersed (prepared tissue blocks into single cell suspension) and in the culture of transmitting cells in the culture of tissue cells. Commonly used digestive solution is Trypsin, EDTA, etc. Tiestase is a mrythinine hydrolytic enzyme. It can cut the carboxyl sideline in the polypeptide chain and the alginine residue in the polypeptide chain. Different cells. The activity of pancreatase decentralized cells is related to the characteristics of tissue or cells, pancreatase concentration, temperature, and action time. When pH 8.0 and 37°C, pancreatase has the strongest role. Therefore temperature and time to avoid causing cell damage to excessive digestion. The working concentration of panicase is generally 0.25%, while the semi -paste wall cells or cells that are sensitive to pancreatase often use low concentration (0.05%) pancreatase for cell digestion. Because EDTA can chelate Ca²⁺ and Mg²⁺, which destroys cell connections to promote the dissipation of cells, a certain amount of EDTA is often added to the pancreatase solution to enhance the dissipation effect.

This product produced by Meilun contains 0.25% trypsin, 0.91mM EDTA and phenol red, dissolved in a calcium-magnesium-free balanced salt solution, filtered and sterilized, and can be directly used for digestion of cultured cells and tissues.

Product Advantage

This product is a modified version. In addition to being convenient, fast, stable and safe, and having good cell status, it also takes less time to digest cells, and is particularly suitable for cells that are difficult to digest, saving you experimental time.

Performance

A549, C6, Hela and Caco-2 cells were digested with MeilunBio MA0232 and international G brand 0.25% Trypsin-EDTA, respectively, and pictures were taken with a microscope to record the digestion process. The results are shown in the figure: the digestion effect of MeilunBio and international G brand Trypsin-EDTA is the same, and it is proved that MeilunBio Trypsin-EDTA has no effect on the cell state in the subsequent passaging culture in the long term application.

Shipping and Storage 

• Storage：Stored at -20°C, valid for one year.
• Shipment：

Usage Statement

Research Use Only (RUO)