Enhanced ATP Assay Kit

Product Description

ATP (Adenosine Triphosphate) serves as the fundamental carrier of energy conversion within biological organisms, and its fluctuation directly correlates with the energy metabolism of various organs. As the most crucial energy molecule, ATP plays a pivotal role in various cellular physiological and pathological processes. Alterations in ATP levels can significantly impact the functionality of many cells. Typically, cellular apoptosis, necrosis, or exposure to certain toxic states lead to a decrease in ATP levels, while stimuli such as high glucose can upregulate intracellular ATP levels in some cells. A decrease in ATP levels usually indicates impaired or decreased mitochondrial function, and during cellular apoptosis, the decline in ATP levels often occurs simultaneously with a decrease in mitochondrial membrane potential. The ATP content determination assay kit can be used to detect ATP levels in common solutions, cells, or tissues.

This assay kit is developed based on the catalysis of firefly luciferase, also known as luciferase, which requires ATP to generate fluorescence. When firefly luciferase and luciferin are both present in excess, fluorescence production within a certain concentration range is directly proportional to the ATP concentration. This enables highly sensitive detection of ATP concentration in solutions.

Product Advantage

• High sensitivity: good detection results in the range of 0.1nM to 100μM.
• High stability: no more than 10% decrease in sample ATP assay results within 30 minutes.
• Good compatibility of prepared samples: Cell or tissue samples lysed with ATP assay lysate in this kit can be used not only for ATP assay, but also for protein concentration detection, SDS-PAGE or some conventional Western assays for more soluble proteins.
• Easy to use: usually 10-20 samples can be measured in 30-60 minutes.
• Simple sample preparation: no need for perchloric acid or trichloroacetic acid (TCA) extraction, the kit provides a special lysis solution, the sample can be detected for ATP assay after simple lysis.

Performance

1. Results of cellular ATP content measurements

Figure 1: Measurement of ATP content after lysis of different cells using MeilunBio and control products

2. Determination of ATP content in ATP solution and Vero cells

Manufacturer	ATP solution (μM)	Vero cell (μM)
MeilunBio Products	1.53	20.27
Control Products	1.47	12.16

Table 1: Measurement of ATP content in ATP solution and Vero cells using MeilunBio and control products.

3. Sample Stability Testing

Figure 2: Stability of ATP content determination after lysing cells with MeilunBio and control products at room temperature for 30 min.

4. The kit has good linearity in the concentration range of 0.01μM-10μM.

Figure 3: Detection effect of this product on ATP standards (0.01μM-10μM)

5. The kit has good linearity in the concentration range of 0.1nM-100nM.

Figure 4: Detection effect of this product on ATP standards (0.1nM-100nM)

6. The lysed sample was used for WB assay (with β-actin as internal reference).Figure 5: Sample for WB after lysis of HL-60 cells MeilunBio and control product (with β-actin as internal reference)

Conclusion

1. From Fig. 1: The ATP content of different cells was detected after lysing with MeilunBio and the control product, and the detection value of MeilunBio product was basically better than that of the control product.
2. From Fig. 2: The stability of MeilunBio product is better than that of the control when the ATP content in the sample is continuously measured for 30 min.
3. From Table 1: The results of meilunBio and the control product are similar when the same concentration of ATP solution is detected; however, when the same number of cells are detected, the results of MeilunBio product are higher and the data are more accurate.
4. From Fig. 3 & 4: MeilunBio products have good linearity in the range of 0.1nM-10μM and 0.1nM-100nM ATP concentration when the standard was added.
5. From Fig. 5: The lysis effect of MeilunBio product was better than that of the control product in WB after lysing HL-60 cells using MeilunBio product and the control product.

Shipping and Storage 

• Storage：Store at -20°C in the dark. The product is valid for 12 months from the date of manufacture.
• Shipment：Transport on dry ice.

Usage Statement

Research Use Only (RUO)