Meilunbio® fg super sensitive ECL luminescence reagent

Product Description

The Meilunbio^® Fg Super Sensitive ECL Luminescence Reagent kit was applied to the WB assay to detect proteins by chemiluminescence. The principle is that horseradish peroxidase (HRP) labeled antibody directly or indirectly binds the target protein on the membrane. After washing the membrane, add the ECL working solution prepared by the kit, which can be catalyzed by HRP.

The minimum detection limit of the antigen in this product can reach the medium femtomolar level. In Western blot experiment, the primary antibody (1 mg/mL) storage solution can be diluted 1:1,000 to 1:50,000 times, and the secondary antibody (1 mg/mL) storage solution can be diluted 1:50,000 to 1:200,000 times.

Product Advantages

• High sensitivity, can detect antigens at medium femtomolar level.
• High signal-to-noise ratio, clear bands.
• Strong anti-quenching ability, repeatable exposure.
• Save antibody, reduce cost.

Guidelines for Use

1. The ECL working solution was prepared by mixing an equal volume of liquid A and liquid B in the ratio of 1:1. Note: The working solution was prepared before use. Use 0.1ml of working solution per cm² of membrane.
2. Take out the washed membrane with tweezers, drain the washing solution on the membrane, set the side of the bound protein facing up, and place it on the clean plastic wrap.
3. Add the ECL working solution, and ensure that the working solution covered the whole membrane during incubation, and incubate for 1-5 minutes at room temperature.
4. Remove the ECL working solution on the membrane, and spread a clean plastic wrap flat on membrane.
5. Expose the blot to film or use your preferred imaging method.

Performance

(Double dilution of the loading protein amount, the target protein Histone H3)

Figure 1: Comparison of the detection sensitivity of MeilunBio ECL reagent with other brands of products of the same grade.

Figure 2: Signal duration of MeilunBio ECL reagent.

Product Notices

1. The kit has high sensitivity, which needs to optimize the loading volume, primary antibody concentration, secondary antibody concentration and dilution solution, membrane and blocking reagent, and according to the protein expression abundance, refer to the recommended antibody dilution ratio, to obtain the best experimental results.
2. If the band on the membrane is too bright, it is recommended to use the membrane regeneration solution to remove the antibody and incubate, and increase the dilution ratio of primary antibody and secondary antibody. If the band is too dark, the secondary antibody dilution ratio can also be reduced to 1:5000 to 1:10,000 times to achieve optimal results.
3. The kit reagent A and reagent B can not pollute each other, otherwise the use effect of the product will be reduced.
4. After incubation with the secondary antibodies, the membrane washing buffer should avoid using sodium azide, which is an inhibitor of HPR.
5. For your safety and health, please wear experimental clothes and disposable gloves.

Shipping and Storage 

• Storage：Store at 2-8℃, A period of 12 months from the date of production.
• Shipment：

Usage Statement

Research Use Only (RUO)