Ham’s F-12K (Kaighn’s) Medium (F-12K)

$100.00

SKU: MA0230 Category:

Product Description

Ham’s F-12K (Kaighn’s) Nutrient Mixture is an improved version of Ham’s F-12 nutrient mixture, based on the formulation by Kaighn. Ham’s F-12 is derived from Ham’s F-10 medium and has increased concentrations of choline, inositol, putrescine, and several amino acids. Ham’s F-12K further enhances the concentrations of amino acids and pyruvate while reducing the glucose content. Additionally, modifications have been made to the composition and concentration of salts (Konigsbergs).

Ham’s F-12K medium was originally designed for culturing primary human liver cells as well as differentiated cells from rats and chickens. It is suitable for single-cell seeding of CHO cells under serum-free conditions and can also be used for certain other types of mammalian cells, such as chondrocytes and rat prostate epithelial cells, when serum is added.

This F-12K (Kaighn’s) Medium is modified as follows:

With Without
L-Glutamine (2mM) HEPES
Sodium Pyruvate (2mM)
Phenol Red (3mg/L)

Please refer to the instruction manual for detailed formulation.

Shipping and Storage 

  • Storage:Store at 2-8°C in the dark for one year.
  • Shipment:

Usage Statement

Research Use Only (RUO)

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Size

500mL