T Cell Expansion Medium, Serum-free, Xeno-free

Product Description

Meiluncell T Cell Expansion Medium, Serum-free, Xeno-free is suitable for promoting and optimizing the activation and efficient expansion of human T lymphocytes in vitro. This product is free of serum and other xenogeneic components, antibiotics and cytokines. Pharmaceutical grade human serum albumin and human transferrin are the only human-sourced components that allow for good growth rates and activity of human T lymphocytes (CD4+, CD8+, polyclonal, or antigen-specific) in allogeneic cell therapy, resulting in safer cells and more consistent results.

Meiluncell T Cell Expansion Medium contains the complete range of nutrients required for T-cell growth. The product contains T-cell basal medium as well as T-cell expansion supplements, which are mixed together prior to use to create a 1× medium.

This product contains: HEPES, phenol red, sodium pyruvate, recombinant human insulin, human transferrin, human serum albumin, etc.. It does not contain: L-glutamine, antibiotics.

Production process: This product is prepared by Water-For-Injection (WFI), and the production procedure is strictly in accordance with the cGMP management regulations, and is completed in the clean room for the record of in vitro diagnostic reagents. The company has obtained ISO9001 quality system certification.

Product Advantage

• No serum, no xenogeneic components, high safety, low batch variation.
• Complete nutrient composition, no need to add additional serum or albumin, which facilitates the translation from research to clinical application and reduces the difficulty of CAR-T drug declaration.
• Supports T-cell activation and expansion culture in vitro and high-density T-cell culture (e.g. >7×106 CD3+ T cells/ml) in static culture.
• Water for injection is formulated in compliance with cGMP regulations for production, and the process is stable.
• Raw materials are selected from self-produced or rigorously tested and purified low endotoxin high purity pharmaceutical grade raw materials, low pyrogen level, reducing exogenous endotoxin interference.
• Strict quality testing procedures, far higher than the required indicators of the same industry, outstanding product performance, indicators comparable to international.

Product Packaging Forms:
PWL098-1: 100mL per bottle;

PWL098-2: 1000mL per bottle.

Performance

1. Proliferation of CTLL-2 cells

Methods: Under the same serum-free conditions, CTLL-2 cells inoculated with the appropriate cell number were cultured using Meiluncell, International G brand, and International L brand T-cell serum-free medium, respectively, and cell counting and viability were examined after 72h.

Results: As shown in Figure 1, CTLL-2 cultured with Meiluncell T-cell serum-free medium proliferated more cells and had a similar survival rate to that of G brand.

Figure 1: Comparison of cell number and viability of CTLL-2 cells cultured in T-cell serum-free medium

2. In vitro expanding fold of human peripheral blood T cells

Methods: PBMC were isolated from human peripheral blood, using T cell serum-free culture medium and 1640+5% FBS of Meiluncell, company  L (international), company  G (international), with or without plasma, respectively. Human peripheral blood T cells were cultured after being activated by CD3/CD28 antibodies. After 14 days, the cell population and survival rate were measured, and the expanding fold was calculated.

Results: With the addition of plasma, human peripheral blood T cells cultured using Meiluncell T cell serum-free cell culture medium can expand >400 times, with a survival rate >90%, which is similar to the control; without the addition of plasma, human peripheral blood T cells cultured using Meiluncell can expand >100 times, and the expanding fold and survival rate are both greater than those of the control. T cells are growing in good condition.

Figure 2: Comparison of cell number and viability of human peripheral blood T cells after 14 days of culture in T cell serum-free medium

Figure 3: Comparison of growth status of human peripheral blood T cells cultured in vitro

3. Growth curve of human peripheral blood T cells cultured in vitro

Methods: PBMC in human peripheral blood were measured using T cell serum-free culture medium and 1640+5% FBS of Meiluncell, company  L (international), company  G (international), with or without plasma, with or without the addition of plasma. Cell growth curves were monitored for 21 days.

Results: Without the addition of plasma, human peripheral blood T cells cultured using Meiluncell product expanded significantly more compared to the control; with the addition of plasma, the growth curve of human peripheral blood T cells cultured using Meiluncell product was similar to the control.

Figure 4: Comparison of 21-day growth curves of human peripheral blood T cells cultured in T cell serum-free medium

4. Expression of cell surface markers of human peripheral blood T cells cultured in vitro

Methods: PBMC were isolated from two sets of human peripheral blood, and cultured after CD3/CD28 antibody activation, using T cell serum-free culture media of Meiluncell, company  G (international), company  L (international) respectively with and without adding plasma. The expression of cell surface markers was detected by flow cytometry on day0 and day14 respectively.

Results: The CD3+ positive rate of human peripheral blood T cells cultured using Meiluncell product was >90%, CD3+/CD4+ remained similar to day0, and the CD3+/CD8+ positive rate was >50%, with no significant difference from the control.

Figure 5: Comparison of surface markers of human peripheral blood T cells after in vitro culture

Shipping and Storage 

• Storage：T-Cell Expansion Basal Medium: Store at 2-8°C protected from light, 12 months

  T-Cell Expansion Supplement: Store at -20°C protected from light, 12 months

  Store the complete culture medium at 2-8°C, protected from light, use within four weeks.

• Shipment：2-8°C

Usage Statement

Research Use Only (RUO)