N-2 Supplement (100×)

Product Description

N-2 Supplement, based on Bottenstein’s N-1 formulation, is a chemically defined serum-free additive primarily recommended for the growth and expression of adult neuronal cell tumors as well as late mitotic neurons of peripheral nervous system (PNS) and central nervous system (CNS) origin. This product is a 100 x working concentration solution, which can be used in conjunction with serum-free neuronal basal medium supplemented with growth factors such as bFGF and EGF, or with DMEM/F-12 medium (PWL005).

Production process: This product is prepared by using Water-For-Injection (WFI), and the production procedure is strictly in accordance with the cGMP management regulations, and is completed in the clean room for the record of in vitro diagnostic reagents. The company has obtained ISO9001 & ISO13485 quality system certification.

Product Advantages

• Supports neuroblastoma cell growth and expansion, ideal supplement for various CNS and PNS neurons.
• Serum-free culture system, optimized formulation.
• Formulated with water for injection, produced in accordance with cGMP regulations, stable process, small batch variation.
• Raw materials are all self-produced or strictly tested and purified low endotoxin high purity pharmaceutical grade raw materials.
• Strict quality testing procedures, much higher than the required indicators in the same industry, the product performance is outstanding, indicators better than imports.
• Low level of pyrogen (endotoxin), reducing exogenous endotoxin interference.

Product packaging form: PWL079-1 is 5mL bottle; PWL079-2 is 50mL bottle.

Performance

1. Detection of proliferation-promoting effect of mouse neural stem cells after combination with B-27

METHODS: Neural stem cells (NSC) extracted from the fetal brain of C57/BL-6 mice were first maintained in culture with medium containing Meiluncell Neural-27 (item no. PWL043) for 3 days, then Meiluncell N-2 supplement and G brand were added, respectively, and continued to be maintained in culture for 5 days for cell counting and survival rate detection.

RESULTS: As shown in Figure 1, the neurospheres cultured with the addition of Meiluncell N-2 proliferated a similar number of cells as the control, with a higher survival rate. As shown in Figure 2, NSC cultured with the addition of Meiluncell N-2 grew well.

Figure 1: Comparison of cell number and survival rate after coaddition of N-2 and B-27 to cultured NSCs

 

Figure 2: Comparison of cell growth status after coaddition of N-2 and B-27 to cultured NSCs

2. Neuroma cell proliferation promotion effect assay

METHODS: PC-12, U251 and C6 were cultured using media supplemented with Meiluncell N-2 and international G brand N-2, respectively, and cell counting and survival rate were detected after 72h.

Results: As shown in Figure 3, the number of proliferating neuroma cells cultured with Meiluncell N-2 was slightly higher, and the survival rate was also slightly higher. As shown in Figure 4, the neuroma cells cultured with Meiluncell N-2 were in good growth condition.

Figure 3: Comparison of cell number and survival rate after addition of N-2 to cultured neuroma cells

Figure 4: Comparison of growth status after addition of N-2 to cultured neuroma cells

Shipping and Storage 

• Storage：Store at -20°C away from light, effective for 12 months.
• Shipment：2-8°C

Usage Statement

Research Use Only (RUO)