Coelenterazine H

Information

Chemical Name: Imidazo[1,2-a]pyrazin-3(7H)-one,6-(4-hydroxyphenyl)2,8-bis(phenylmethyl)

CAS No.: 50909-86-9

Molecular Structure:

Molecular Formula:C₂₆H₂₁N₃O₂

Molecular Weight：407.46

Purity: >98%,HPLC

Appearance: Light yellow to off-white powder

Solubility(at 25°C): Ethanol

Storage Condition:-20°C sealed storage, away from moisture and light

Shipment: 

Description

Coelenterazine(Item No. MB2562)  is the most abundant naturally occurring luciferin in nature and is the light energy storage molecule for the vast majority of marine luminescent organisms (more than 75%). Coelenterazine serves as a substrate for many luciferase enzymes, such as Renal luciferase, Gaussia secretory luciferase, as well as photoproteins including aequorin and obelia. The principle of luminescence is that luciferase: which uses coelenterazine as a substrate, oxidizes coelenterazine in the presence of molecular oxygen, generating high-energy intermediates and emitting blue light in the process, with a peak emission wavelength of about 450-480 nm. unlike the luciferin/luciferase system of the beetles (or fireflies), the coelenterazine/luciferase system does not require ATP, and is therefore more convenient for in vivo studies. biofluorescence studies.

Application：

1. Coelenterazine is often used as a luminescent substrate for reporter gene assays based on fluorescence analysis as well as in vivo animal assays;
2. Aequorin can also autofluoresce in an enzyme-independent oxidative system, and superoxide anion and peroxynitrite anion in cells and tissues can enhance the autofluorescence signal, so it can also be used to detect the level of reactive oxygen species (ROS) in cells/tissues.
3. More importantly, the Aequorin complex consists of a 22kDa Apoaequorin protein, molecular oxygen and the substrate luminalin. It is only when calcium ions (Ca2+) bind to the complex that coelenterazine can be oxidized to produce the high-energy product coelenteramide, which simultaneously releases CO2 and blue fluorescence (~466 nm). It is this Ca2+-dependent reaction process that makes coelenterazine ideally suited for monitoring calcium levels in living cells.

Usage Statement

Research Use Only (RUO)