Information
Chemical Name: 2-(3,6-diacetoxy-2,7-dichloro-9h-xanthen-9-yl) benzoic acid
CAS No.: 4091-99-0
Molecular Structure: 
Molecular Formula: C24H16Cl2O7
Molecular Weight: 487.29
Purity: ≥97%, BR
Ex/Em (nm): 504/529
Solubility (at 25℃): DMSO: ≥ 150 mg/mL
Storage Condition: Store in a tightly closed container and dry place, protect from light, -20℃
Shipment:
Description
2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA) (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm.
Usage Statement
Research Use Only (RUO)
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